Researchers isolated LAB . strains Lactobacillus brevisAnd the Lactobacillus plantsFor therapeutic activity against oral pathogens Porphyromonas gingivalis (P. gingivalis), It is considered the backbone of periodontal diseaseAnd the Decreases in biofilm formation were observed after dental treatment. The pathogen is known to stimulate the production of biofilms implicated in the development of the disease.
“The ability of LAB CFS to disrupt biofilm formation activity at various concentrations is critical to preventing recolonization s. gums As the protective biofilm layer in the pocket is destroyed by the treatment,” Study authors write Oral biology.
“to. short And the to. the plants Showed excellent antimicrobial and anti-biofilm activityP. gingivalis. “
Shift in the microbial balance
Periodontal disease is a chronic dental problem that affects nearly 50% of adults and adolescents in developing countries and P. gumsIt is one of the major microbes associated with disease pathogenesis and possesses a number of virulent factors, such as lipopolysaccharide (LPS) layer in cell walls, inactivation of adhesion, biofilm formation, and genipins for tissue degradation.
“s. gums It is the primary pathogen that alters the balance of the oral microbiota and ultimately disrupts the regulation of the immune response pathway. Transformation in the oral microbiota leads to dysbacteriosis after invasion s. gums. “
Antibiotics and antibacterial mouthwash are recommended to prevent colonization after dental treatment, but the biofilm caused by P. gumsThe effectiveness of both antibiotics and mouthwash reduces tooth stains and reduces palate sensitivity.
The effect of microorganisms
Probiotics modulate immune responses to dental infections and the production of antimicrobial compounds, thereby preventing the spread of pathogens and thus the formation of biofilms.
“Probiotics are a promising alternative for improving oral health, especially in the management of periodontal disease and periodontal disease.” The authors keep.
They explain that some strains are already in use to treat many dental problems, and studies indicate their potential as an adjunct therapy to reduce periodontal pathogens and improve clinical outcomes in the case of non-surgical treatment.
Several studies have found that periodontal pathogens can be inhibited by live culture of probiotics or cell-free supernatant (CFS) from different commercial strains.
“Furthermore, the rise in antibiotic resistance highlights the critical need for alternative treatment options.”
Lactobacillus brevisAnd the Lactobacillus plantsIt was isolated from fermented tapioca and its potential in oral health was evaluated. LABs were screened for antimicrobial sensitivity and anti-biofilm properties and evaluated for probiotic potential. The results were compared with the commercial strain Lactobacillus rhamnosus.
The cell-free supernatant (CFS) of the isolated probiotics was shown.excellentAntimicrobial and anti-biofilm activity against P. gumsIt was comparable with commercial control, L. rhamnosus. This supports findings from another similar study and indicates that LABs have the potential to behostile against periodontal pathogens,Authors comment.
Furthermore, the commercial strain possesses inhibitory activity against many other periodontal pathogens, including F. nucleusAnd the actinomycetes.
The mechanism of hostile activity related to . has been hypothesized Competitive interactionand secretion of antimicrobial substances and other biologically active compounds.
Biofilm testing showed that CFS from the study of probiotics and the commercial variant disrupted biofilm formation and helped eliminate most P. gumspathogenesis by interfering with gene activity.
Researchers say that: Production of potent antimicrobial substances that can disrupt biofilm has demonstrated that LAB CFS isolated from fermented foods is highly competent in combating colonization and biofilm formation. s. gums.
“There is also the possibility that metabolites produced by the isolated probiotics could affect cell wall synthesis, cell metabolism, and cellular attachments.”
They further confirm that the production of organic acids may contribute to the antimicrobial activity of the tested LAB CFS. Although neutralized L. in shortAnd the L plants.showed no inhibition activity L. rhamnosusshowed slight inhibition towards the offending pathogen.
Antimicrobial mechanisms neutralize the cytoplasmic membrane of bacterial cells, causing them to rupture and interfere with “quorum awareness“It is a pathogen, they add.
The auto-aggregation and aggregation activity of LABs has been considered in preventing biofilm formation. It was observed that both L. in shortAnd the L plants.Promote spontaneous activity and aggregation to help prevent bacteria from attaching to gum tissue and enhance the action of probiotics.
Both strains were tolerant of alpha-amylase and resistant to the analytic properties of oral saliva. Finally, only a slight decrease in pH was observed in the presence of sugar supplementation, indicating that no elevated acid production was initiated and thus causal dental caries.
source: Archives Oral biology
Published online, August 19, 2022: doi: 10.1016 / j.archoralbio.2022.105515
“Characterization and properties of potential oral probiotics Lactobacillus planta FT 12 And the Lactobacillus brevis fit 6 isolated from Malaysian fermented food
Authors: N.S. Mohd-Zubri, et al.